gr 1 a488 Search Results


alexa fluor 488 conjugated anti ly6g  (Bioss)
90
Bioss alexa fluor 488 conjugated anti ly6g
Male C57BL/6 mice were pretreated with either AA (30 mg/kg) or vehicle 1 h before hepatic I/R surgery. After 6 h reperfusion, liver tissues and serum samples were harvested. (A) Representative histological staining (H&E) of ischemic liver tissue. Results representative of 4-6 mice/group. Liver damage, evaluated by Suzuki’s histological score. Scale bar: 30μm. (B) Hepatocellular function in serum samples was evaluated by sALT and sAST levels (IU/L). Results expressed as mean±SD (n=4-6 samples/group). (C) & (D) Liver neutrophils and monocytes/macrophages were detected by immunofluorescent staining. Quantification of <t>Ly6G+</t> cells and CD11b+ cells per HPF. Representative of 4-6 mice/group. Scale bar: 30μm. (E) RT-qPCR for detection of IL-6, TNF-α, and CXCL1 in ischemic livers (n=4-6 samples/group). * Significant difference ( P < 0.05) compared with corresponding control. # Significant difference ( P < 0.05) compared with I/R.
Alexa Fluor 488 Conjugated Anti Ly6g, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 488 conjugated anti ly6g/product/Bioss
Average 90 stars, based on 1 article reviews
alexa fluor 488 conjugated anti ly6g - by Bioz Stars, 2026-03
90/100 stars
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gr 1 a488  (R&D Systems)
92
R&D Systems gr 1 a488
Male C57BL/6 mice were pretreated with either AA (30 mg/kg) or vehicle 1 h before hepatic I/R surgery. After 6 h reperfusion, liver tissues and serum samples were harvested. (A) Representative histological staining (H&E) of ischemic liver tissue. Results representative of 4-6 mice/group. Liver damage, evaluated by Suzuki’s histological score. Scale bar: 30μm. (B) Hepatocellular function in serum samples was evaluated by sALT and sAST levels (IU/L). Results expressed as mean±SD (n=4-6 samples/group). (C) & (D) Liver neutrophils and monocytes/macrophages were detected by immunofluorescent staining. Quantification of <t>Ly6G+</t> cells and CD11b+ cells per HPF. Representative of 4-6 mice/group. Scale bar: 30μm. (E) RT-qPCR for detection of IL-6, TNF-α, and CXCL1 in ischemic livers (n=4-6 samples/group). * Significant difference ( P < 0.05) compared with corresponding control. # Significant difference ( P < 0.05) compared with I/R.
Gr 1 A488, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gr 1 a488/product/R&D Systems
Average 92 stars, based on 1 article reviews
gr 1 a488 - by Bioz Stars, 2026-03
92/100 stars
  Buy from Supplier
gr 1 a488  (Thermo Fisher)
86
Thermo Fisher gr 1 a488
Male C57BL/6 mice were pretreated with either AA (30 mg/kg) or vehicle 1 h before hepatic I/R surgery. After 6 h reperfusion, liver tissues and serum samples were harvested. (A) Representative histological staining (H&E) of ischemic liver tissue. Results representative of 4-6 mice/group. Liver damage, evaluated by Suzuki’s histological score. Scale bar: 30μm. (B) Hepatocellular function in serum samples was evaluated by sALT and sAST levels (IU/L). Results expressed as mean±SD (n=4-6 samples/group). (C) & (D) Liver neutrophils and monocytes/macrophages were detected by immunofluorescent staining. Quantification of <t>Ly6G+</t> cells and CD11b+ cells per HPF. Representative of 4-6 mice/group. Scale bar: 30μm. (E) RT-qPCR for detection of IL-6, TNF-α, and CXCL1 in ischemic livers (n=4-6 samples/group). * Significant difference ( P < 0.05) compared with corresponding control. # Significant difference ( P < 0.05) compared with I/R.
Gr 1 A488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gr 1 a488/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
gr 1 a488 - by Bioz Stars, 2026-03
86/100 stars
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cgr19 polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
CGR19 is involved in the negative regulation of cell proliferation and cell cycle arrest in response to stress.
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alexa fluor 488 anti mouse ly 6g ly 6c gr 1  (BioLegend)
N/A
Alexa Fluor 488 anti mouse Ly 6G Ly 6C Gr 1 RB6 8C5 Isotype Rat IgG2b κ Reactivity Mouse Apps FC IHC Size 100 μg
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ly-6g polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow
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egr1 polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
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fcgr1a cd64 polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
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rat anti mouse gr 1  (Bio-Rad)
N/A
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mtgr1 polyclonal antibody, alexa fluor 488 conjugated  (Bioss)
N/A
May function as a complex with the chimeric protein RUNX1/AML1-CBFA2T1/MTG8 which is produced in acute myeloid leukemia with the chromosomal translocation t(8;21). May thus be involved in the repression of AML1-dependent transcription and the induction
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Male C57BL/6 mice were pretreated with either AA (30 mg/kg) or vehicle 1 h before hepatic I/R surgery. After 6 h reperfusion, liver tissues and serum samples were harvested. (A) Representative histological staining (H&E) of ischemic liver tissue. Results representative of 4-6 mice/group. Liver damage, evaluated by Suzuki’s histological score. Scale bar: 30μm. (B) Hepatocellular function in serum samples was evaluated by sALT and sAST levels (IU/L). Results expressed as mean±SD (n=4-6 samples/group). (C) & (D) Liver neutrophils and monocytes/macrophages were detected by immunofluorescent staining. Quantification of Ly6G+ cells and CD11b+ cells per HPF. Representative of 4-6 mice/group. Scale bar: 30μm. (E) RT-qPCR for detection of IL-6, TNF-α, and CXCL1 in ischemic livers (n=4-6 samples/group). * Significant difference ( P < 0.05) compared with corresponding control. # Significant difference ( P < 0.05) compared with I/R.

Journal: Oncotarget

Article Title: Asiatic acid protects against hepatic ischemia/reperfusion injury by inactivation of Kupffer cells via PPARγ/NLRP3 inflammasome signaling pathway

doi: 10.18632/oncotarget.21151

Figure Lengend Snippet: Male C57BL/6 mice were pretreated with either AA (30 mg/kg) or vehicle 1 h before hepatic I/R surgery. After 6 h reperfusion, liver tissues and serum samples were harvested. (A) Representative histological staining (H&E) of ischemic liver tissue. Results representative of 4-6 mice/group. Liver damage, evaluated by Suzuki’s histological score. Scale bar: 30μm. (B) Hepatocellular function in serum samples was evaluated by sALT and sAST levels (IU/L). Results expressed as mean±SD (n=4-6 samples/group). (C) & (D) Liver neutrophils and monocytes/macrophages were detected by immunofluorescent staining. Quantification of Ly6G+ cells and CD11b+ cells per HPF. Representative of 4-6 mice/group. Scale bar: 30μm. (E) RT-qPCR for detection of IL-6, TNF-α, and CXCL1 in ischemic livers (n=4-6 samples/group). * Significant difference ( P < 0.05) compared with corresponding control. # Significant difference ( P < 0.05) compared with I/R.

Article Snippet: Alexa Fluor 488-conjugated anti-Ly6G, Alexa Fluor 488-conjugated anti-F4/80 and Alexa Fluor 594-conjugated anti-CD11b were purchased from Bioss, China.

Techniques: Staining, Quantitative RT-PCR

Mice were pretreated with GW9662 (2 mg/kg, i.p.) or vehicle 30 min prior to treatment with AA, followed by an I/R insult. Liver tissues and serum samples were harvested 6 h after reperfusion. (A) Representative histological staining, Ly6G+ cells and CD11b+ cells infiltration and immunohistochemical staining of NLRP3 in ischemic livers. Scale bar: 30μm. (B) Suzuki’s histological score and sALT. (C) Quantitative analysis of infiltrated Ly6G+ cells and CD11b+ cells. (D) mRNA levels of IL-6, TNF-α and CXCL1, as well as mRNA levels of NLRP3 and IL-1β were determined using RT-qPCR. (E) Protein expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1 p10, pro-IL-1β, IL-1β, PPARγ, Bcl-2, pro-caspase-3 and caspase-3 were detected using Western blot analysis. (F) Quantitative analysis of NLRP3 positively-stained cells. (G) ELISA analysis of IL-1β levels in animal serums. (H) Co-immunoprecipitation and Western blot for assessing the NLRP3 inflammasome assembly. The results are presented as the mean±SD of 4-6 animals per group. Blots shown are representative of 3 experiments with similar results. * P < 0.05 compared between the indicated groups. ** P < 0.01 compared between the indicated groups.

Journal: Oncotarget

Article Title: Asiatic acid protects against hepatic ischemia/reperfusion injury by inactivation of Kupffer cells via PPARγ/NLRP3 inflammasome signaling pathway

doi: 10.18632/oncotarget.21151

Figure Lengend Snippet: Mice were pretreated with GW9662 (2 mg/kg, i.p.) or vehicle 30 min prior to treatment with AA, followed by an I/R insult. Liver tissues and serum samples were harvested 6 h after reperfusion. (A) Representative histological staining, Ly6G+ cells and CD11b+ cells infiltration and immunohistochemical staining of NLRP3 in ischemic livers. Scale bar: 30μm. (B) Suzuki’s histological score and sALT. (C) Quantitative analysis of infiltrated Ly6G+ cells and CD11b+ cells. (D) mRNA levels of IL-6, TNF-α and CXCL1, as well as mRNA levels of NLRP3 and IL-1β were determined using RT-qPCR. (E) Protein expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1 p10, pro-IL-1β, IL-1β, PPARγ, Bcl-2, pro-caspase-3 and caspase-3 were detected using Western blot analysis. (F) Quantitative analysis of NLRP3 positively-stained cells. (G) ELISA analysis of IL-1β levels in animal serums. (H) Co-immunoprecipitation and Western blot for assessing the NLRP3 inflammasome assembly. The results are presented as the mean±SD of 4-6 animals per group. Blots shown are representative of 3 experiments with similar results. * P < 0.05 compared between the indicated groups. ** P < 0.01 compared between the indicated groups.

Article Snippet: Alexa Fluor 488-conjugated anti-Ly6G, Alexa Fluor 488-conjugated anti-F4/80 and Alexa Fluor 594-conjugated anti-CD11b were purchased from Bioss, China.

Techniques: Staining, Immunohistochemical staining, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Immunoprecipitation

Mice were pretreated with GdCl3 24 h prior to AA treatment or AA+GW9662 treatment, followed by an I/R insult. Liver tissues and serum samples were harvested after 6 h reperfusion. (A) Representative histological staining, Ly6G+ cells and CD11b+ cells infiltration and immunohistochemical staining of NLRP3 in ischemic livers. Scale bar: 30μm. (B) Suzuki’s histological score and sALT. (C) Quantitative analysis of infiltrated Ly6G+ cells and CD11b+ cells. (D) mRNA levels of IL-6, TNF-α and CXCL1, as well as mRNA levels of NLRP3 and IL-1β were determined using RT-qPCR. (E) Protein expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1 p10, pro-IL-1β, IL-1β, PPARγ, Bcl-2, pro-caspase-3 and caspase-3 were detected using Western blot analysis. (F) Quantitative analysis of NLRP3 positively-stained cells. (G) ELISA analysis of IL-1β levels in animal serums. The results are presented as the mean±SD of 4-6 animals per group. Blots shown are representative of 3 experiments with similar results. * P < 0.05 compared between the indicated groups. ** P < 0.01 compared between the indicated groups.

Journal: Oncotarget

Article Title: Asiatic acid protects against hepatic ischemia/reperfusion injury by inactivation of Kupffer cells via PPARγ/NLRP3 inflammasome signaling pathway

doi: 10.18632/oncotarget.21151

Figure Lengend Snippet: Mice were pretreated with GdCl3 24 h prior to AA treatment or AA+GW9662 treatment, followed by an I/R insult. Liver tissues and serum samples were harvested after 6 h reperfusion. (A) Representative histological staining, Ly6G+ cells and CD11b+ cells infiltration and immunohistochemical staining of NLRP3 in ischemic livers. Scale bar: 30μm. (B) Suzuki’s histological score and sALT. (C) Quantitative analysis of infiltrated Ly6G+ cells and CD11b+ cells. (D) mRNA levels of IL-6, TNF-α and CXCL1, as well as mRNA levels of NLRP3 and IL-1β were determined using RT-qPCR. (E) Protein expressions of NLRP3, ASC, pro-caspase-1, cleaved caspase-1 p10, pro-IL-1β, IL-1β, PPARγ, Bcl-2, pro-caspase-3 and caspase-3 were detected using Western blot analysis. (F) Quantitative analysis of NLRP3 positively-stained cells. (G) ELISA analysis of IL-1β levels in animal serums. The results are presented as the mean±SD of 4-6 animals per group. Blots shown are representative of 3 experiments with similar results. * P < 0.05 compared between the indicated groups. ** P < 0.01 compared between the indicated groups.

Article Snippet: Alexa Fluor 488-conjugated anti-Ly6G, Alexa Fluor 488-conjugated anti-F4/80 and Alexa Fluor 594-conjugated anti-CD11b were purchased from Bioss, China.

Techniques: Staining, Immunohistochemical staining, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay